Research on phase diversity method based on L-BFGS for fluorescence microscopy in adaptive optics

HUANG Zelu; LI Xin; SU Delei; YU Wenhao; ZHANG Zhiguang

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Research on phase diversity method based on L-BFGS for fluorescence microscopy in adaptive optics

Optical Information Technology | 更新时间：2026-01-05

- Research on phase diversity method based on L-BFGS for fluorescence microscopy in adaptive optics
- OPTICAL TECHNIQUE Vol. 51, Issue 6, Pages: 649-655(2025)
- 作者机构：
  
  江苏海洋大学电子工程学院，江苏连云港 222005
- 作者简介：
- 基金信息：
- DOI：
  CLC： TP273.2
- Received：07 April 2025，
  
  Revised：2025-08-09，
  
  Published：20 November 2025
- 稿件说明：
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黄则陆,李欣,苏德磊等.基于有限内存拟牛顿法的荧光显微成像相位差异自适应光学技术研究[J].光学技术,2025,51(06):649-655.

HUANG Zelu,LI Xin,SU Delei,et al.Research on phase diversity method based on L-BFGS for fluorescence microscopy in adaptive optics[J].OPTICAL TECHNIQUE,2025,51(06):649-655.
黄则陆,李欣,苏德磊等.基于有限内存拟牛顿法的荧光显微成像相位差异自适应光学技术研究[J].光学技术,2025,51(06):649-655. DOI：

HUANG Zelu,LI Xin,SU Delei,et al.Research on phase diversity method based on L-BFGS for fluorescence microscopy in adaptive optics[J].OPTICAL TECHNIQUE,2025,51(06):649-655. DOI：

摘要

荧光显微镜因其对样品损伤小、可进行特异性标记等特点，被广泛应用于细胞结构及动态过程的研究。然而当光的波前由于系统自身缺陷或样品的折射效应而发生畸变时，这些光学像差的产生会大大降低成像的对比度和分辨率。自适应光学（AO）是一种校正波前畸变的技术，将自适应光学技术与荧光显微镜相结合，可以有效提高成像的质量。文章提出了一种基于有限内存拟牛顿法（L-BFGS）的荧光显微成像相位差异（PD）自适应光学技术，相位差异自适应光学技术最初用于天文观测领域，在生物样本显微成像方面的应用尚未得到充分探索。仿真与实测结果表明，相比基于高斯牛顿法（Gauss-Newton）的荧光显微相位差异自适应光学技术，方法在计算速度、波前重建能力方面都有着较大的优势。研究结果可为恢复生物细胞的衍射极限成像提供重要的理论基础。

Abstract

Fluorescence microscopy is widely used in the study of cell structures and dynamic processes due to its minimal sample damage and ability to perform specific labeling. However

when the light wavefront is distorted due to system defects or refractive effects of the sample

these optical aberrations can significantly reduce imaging contrast and resolution. Adaptive optics （AO） is a technology used to correct wavefront distortions. By combining adaptive optics with fluorescence microscopy

imaging quality can be effectively improved. The fluorescence microscopy phase diversity （PD） adaptive optics technique based on the limited-memory Broyden-Fletcher-Goldfarb-Shanno （L-BFGS） method is proposed. Phase diversity adaptive optics was initially used in astronomical observations

and its application in biological sample microscopy has not been fully explored. Simulation and experimental results show that

compared to the fluorescence microscopy phase diversity adaptive optics technique based on the Gauss-Newton method

the proposed method offers significant advantages in computational speed and wavefront reconstruction capabilities. The research results provide an important theoretical foundation for restoring diffraction-limited imaging of biological cells.

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references

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