大视场dPCR检测系统中的荧光LED照明设计

金旭东; 张磬瀚; 严言; 郑继红; 韦晓孝; 万新军

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大视场dPCR检测系统中的荧光LED照明设计

光学器件与系统设计 | 更新时间：2025-10-09

- 大视场dPCR检测系统中的荧光LED照明设计
- Design of the fluorescent LED illumination in a Wide-field dPCR detection system
- 光学技术 2025年51卷第4期页码：424-431
- 作者机构：
  
  1.上海理工大学光电信息与计算机学院，上海 200093
  2.上海理工大学上海市现代光学系统重点实验室，上海 200093
- 作者简介：
  
  金旭东（2000— ），男，硕士研究生，从事光学系统设计研究。2240122624@qq.com
  郑继红（1975— ），女，教授，博士，从事光学系统、电光器件、PDLC材料和信息光学等方面的研究。jihongzheng@sina.com
- 基金信息：
  
  上海市优秀学术/技术带头人计划(22XD1401000)
- DOI：
  中图分类号： TH742
- 收稿：2024-11-19，
  
  修回：2025-02-27，
  
  纸质出版：2025-07-20
- 稿件说明：
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金旭东,张磬瀚,严言等.大视场dPCR检测系统中的荧光LED照明设计[J].光学技术,2025,51(04):424-431.

JING Xudong,ZHANG Qinhan,YAN Yan,et al.Design of the fluorescent LED illumination in a Wide-field dPCR detection system[J].OPTICAL TECHNIQUE,2025,51(04):424-431.
金旭东,张磬瀚,严言等.大视场dPCR检测系统中的荧光LED照明设计[J].光学技术,2025,51(04):424-431. DOI：

JING Xudong,ZHANG Qinhan,YAN Yan,et al.Design of the fluorescent LED illumination in a Wide-field dPCR detection system[J].OPTICAL TECHNIQUE,2025,51(04):424-431. DOI：

摘要

数字聚合酶链式反应（dPCR）是一种能实现绝对定量检测的分子生物学技术。传统的dPCR荧光检测系统在检测通量、检测速度、系统体积以及成本之间难以达到合理平衡。特别是传统照明系统设计在照射面积、均匀性、体积和开关响应速度方面存在限制，影响设备的整体性能及应用。为此，本文设计了一种基于匀光设计LED激发优化的便携式dPCR大视场荧光检测系统，旨在不牺牲检测速度与通量的前提下，缩小系统体积并降低成本。系统通过高集成度的环形LED光源排布，实现了18mm×18mm全视场范围内三种荧光通道（FAM、HEX、ROX）的均匀照明，实际照明均匀度超过90%，并支持三通道快速开关切换。相比于传统的照明模块，LED匀光激发照明在小型化、低成本以及高集成度方面具有优势，适应于多种荧光检测应用场景，促进dPCR技术未来的发展。

Abstract

Digital Polymerase Chain Reaction （dPCR） is a molecular biology technique that enables absolute quantification. However

conventional dPCR fluorescence detection systems face challenges in achieving an optimal balance among detection throughput

speed

system size

and cost. In particular

traditional illumination system designs are constrained by limitations in illumination area

uniformity

volume

and switching response

which affect the overall performance and applicability of the equipment. To address these issues

this study proposes a portable dPCR fluorescence detection system optimized for large-field imaging based on an LED excitation design with uniform illumination. This system aims to reduce size and costs without compromising detection speed and throughput. Utilizing a high-integration ring-shaped LED light source arrangement

the system achieves uniform illumination across an 18 mm × 18 mm field of view for three fluorescence channels （FAM

HEX

ROX）

with actual illumination uniformity exceeding 90%

and supports rapid three-channel switching. Compared with traditional illumination modules

the LED-based uniform excitation illumination offers advantages in miniaturization

cost-efficiency

and integration

making it suitable for various fluorescence detection applications and advancing the future development of dPCR technology.

关键词

Keywords

references

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