具有大成像深度高层析能力的高分辨率共聚焦显微物镜设计

葛亚雯; 杨照清; 薛萌; 郭汉明

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具有大成像深度高层析能力的高分辨率共聚焦显微物镜设计

光学器件与系统设计 | 更新时间：2026-01-22

- 具有大成像深度高层析能力的高分辨率共聚焦显微物镜设计
- Design of high resolution confocal microscope objective with large imaging depth and high tomographic ability
- 光学技术 2026年52卷第1期页码：12-17
- 作者机构：
  
  1.上海理工大学光电信息与计算机工程学院，上海 200093
  2.上海理工大学上海市现代光学系统重点实验室，上海 200093
- 作者简介：
  
  葛亚雯（1999— ），女，硕士研究生，从事光学仪器设计方面的研究。gyw17852582517@163.com
  郭汉明（1977— ），男，教授，博士，从事超分辨光学显微成像和光谱技术方面的研究。hmguo@usst.edu.cn
- 基金信息：
  
  上海市地方能力建设项目(2101502900)
- DOI：
  中图分类号： TN202
- 收稿：2025-10-24，
  
  修回：2025-12-04，
  
  纸质出版：2026-01-20
- 稿件说明：
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葛亚雯,杨照清,薛萌等.具有大成像深度高层析能力的高分辨率共聚焦显微物镜设计[J].光学技术,2026,52(01):12-17.

GE Yawen,YANG Zhaoqing,XUE Meng,et al.Design of high resolution confocal microscope objective with large imaging depth and high tomographic ability[J].OPTICAL TECHNIQUE,2026,52(01):12-17.
葛亚雯,杨照清,薛萌等.具有大成像深度高层析能力的高分辨率共聚焦显微物镜设计[J].光学技术,2026,52(01):12-17. DOI：

GE Yawen,YANG Zhaoqing,XUE Meng,et al.Design of high resolution confocal microscope objective with large imaging depth and high tomographic ability[J].OPTICAL TECHNIQUE,2026,52(01):12-17. DOI：

摘要

针对在体皮肤光学层析成像中MEMS振镜扫描系统与高层析能力物镜的匹配难题，设计了一款工作波段为830nm的高分辨率共聚焦显微物镜，旨在克服因生物组织球差导致的成像深度受限与跨介质像差平衡问题。设计采用基于皮肤多层光学模型的多目标优化方法，在2mm入瞳直径的约束下，实现了成像深度达到300μm，横向分辨率优于0.83μm，轴向分辨率优于4.05μm，全视场波前误差优于λ/16，在皮肤表面及各组织深度处的MTF均与衍射极限紧密贴合。该设计通过多深度像差协同优化

为便携式在体皮肤光学层析成像系统提供了可靠的光学解决方案。

Abstract

Aiming at the matching problem between the MEMS galvanometer scanning system and the high-resolution objective lens in in vivo skin optical tomography

a high-resolution confocal microscope objective with a working band of 830 nm is designed to overcome the imaging depth limitation and cross-media aberration balance caused by the spherical aberration of biological tissue. The design adopts a multi-objective optimization method based on the skin multi-layer optical model. Under the constraint of 2mm entrance pupil diameter

the imaging depth reaches 300μm

the lateral resolution is better than 0.83μm

the axial resolution is better than 4.05μm

and the full-field wavefront error is better than λ / 16. The MTF at the skin surface and the depth of each tissue is closely fitted with the diffraction limit. This design provides a reliable optical solution for portable in vivo skin optical tomography system through multi-depth aberration collaborative optimization.

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references

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